This environmentaly friendly mode of transport is now experiencing a comeback and since 2024 there are two trolleybus lines in service, numbers 58 and 59. These lines operate from 05:00 until 24:00.
All trolleybuses are low floor.
Current bus line 131
Bořislavka – U Matěje – Zelená – Hradčanská

Current bus line 137
Na Knížecí – Santoška – Malvazinky – U Waltrovky

Current bus line 176
Karlovo náměstí – Švandovo divadlo – Holečkova – Stadion Strahov

Trolleybus line 58 (former bus line 140)
Palmovka – Prosek – Letňany – OC Čakovice – Čakovice – Miškovice

Status: In service.
Trolleybus line 59 (former bus line 119)
Nádraží Veleslavín – Divoká Šárka – K Letišti – Letiště

Status: In service since March 6th 2024.